A fluorimetric method for red blood cell sorbitol dehydrogenase activity.
نویسندگان
چکیده
A new fluorimetric method for the quantification of red blood cell (RBC) sorbitol dehydrogenase is described. It is based on the oxidation of sorbitol to fructose, in presence of NAD+, catalysed by the RBC-sorbitol dehydrogenase. The quantity of NADH formed is then measured in a filter fluorimeter. Comparison with an indirect spectrophotometric assay yielded good correlation; however, the present method offers several advantages: it is more rapid, simple and inexpensive. It should be useful to screen for sorbitol dehydrogenase deficiency in large numbers of individuals, particularly patients with diabetes or cataracts.
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عنوان ژورنال:
- Journal of clinical pathology
دوره 36 6 شماره
صفحات -
تاریخ انتشار 1983